亲爱的大家，
我来自缅甸——世界上30个结核病负担最重的国家之一。最近，我打算用Stool Xpert MTB/ RIF测试对疑似肺结核儿童进行研究。我知道有很多关于这方面的论文，我也读过其中一些。
但我想要求任何有经验的Stool Xpert MTB/ RIF测试的人分享测试经验和结果。然后，我想知道它是否真的对儿童结核病的确诊有效。此外，我认为阳性率和敏感性可能不是很高。
>再次感谢。
期待您的经验分享!
Aung Phyo Khaing
高级结核病实验室主管
WHO外派人员到国家结核病规划，缅甸
By Aung Phyo Khaing on Thu, ju2019 23:36:48 -0400
回复: MGIT培养涂片固定方法
你好，请尝试2%至5%的牛白蛋白作为固定剂
-
*Dr。基肖尔Reddy VC。** PhD*
*微生物学家*
International Center for Excellence in Laboratory Training-ICELT
By Kishore Reddy VC on Wed, 10 ju7 2019 00:27 . 02 -0400
Reply to: MGIT培养涂片固定方法
——
By Mengistu Tadesse on Tue, 9 Jul 2019 22:16:26 -0400
在我们的实验室，当我们从MGIT阳性培养物中做涂片时，它们在染色过程中被完全洗掉。我们用隔热板在75度的温度下固定两个小时。但这似乎没有帮助。我们以前也试过纯牛奶，但效果不太好。有什么问题吗?(我们在使用之前要清洗切片，以确保没有油的残留物)。
你们实验室还用什么固定剂?
By Ranjan Perera on Tue, 9 ju2019 16:35:47 -0400
By Mercedes Perez Gonzalez on Tue, 9 Jul 2019 08:00:36 -0400
仅供参考，并对交叉发布的消息表示歉意：-转发消息----------------发件人：Laura Lopez Gonzalez日期：2019年7月9日星期二凌晨4:24主题：关于TB Lam试验的优点和局限性的新观点文章
收件人：<>
大家好，
这篇评论文章以一种
非常容易理解的方式为艾滋病毒高负担国家的人阐述了该测试的好处和局限性。
经事先批准后，也可通过
，
--
劳拉•洛佩兹•冈萨雷斯
+27（0）76.467.5208（手机）
劳拉•洛佩兹•冈萨雷斯1（Skype）
@LLopezGonzalez
*TB：这个尿检可以救你的命*
https://bhekisisa.org/article/2019-07-04-this-new-test-will-detect-tb-in-your-pee/如果诊断南非的最致命的疾病是像
药店怀孕测试一样简单？那一天可能比你想象的要近。*
*Keertan Dheda教授*
“白瘟疫”，在南非的金矿上，
肺结核-无论它叫什么名字，这种细菌早在现代科学称之为肺结核之前就已经四处游荡了。
1882年3月24日，科学家罗伯特·科赫发现了引起结核病的病菌，并将其命名为结核分枝杆菌，
，
只有通过科赫发明的新型
染色技术，才有可能使结核病，然后霍乱，在显微镜下活过来。
一个多世纪以来，结核病检测就是这样的：一排排
科学家在全世界的实验室里俯身在显微镜下，
绝望地在泥泞和泥泞中寻找那些微小的棒状物，人们可以把它们作为痰样本的一部分来咳嗽。
然后在2010年，世界卫生组织（WHO）批准了基因专家
<；https://bhekisisa.org/article/2017-05-08-00-is-all-in-one-healthcare-a-dream/>
-
咖啡机大小的机器，可以在两个
小时内提供结核病诊断。到2011年，这种疾病的快速检测领域不断扩大，据世界卫生组织称，仅在这一年，就将影响到800多万人。
，这些
测试都依赖于病人从呼吸道深处产生痰的能力。世卫组织说，南非每年有38000名儿童患上活动性肺结核，对他们中的大多数人来说，这是一项太困难的任务；https://www.who.int/tb/data/GTBreportCountryProfiles.pdf?ua=1>.
根据世卫组织最新报告，许多艾滋病毒携带者约占该国结核病死亡人数的70%
<；https://www.who.int/tb/publications/global_report/en/> — 同时发现
产生的痰具有挑战性。
这种间隙可能是致命的。2015年，一项对30多项研究和
对
全球南部地区感染艾滋病毒的成人和儿童进行的3000多例尸检的回顾发现，结核病导致了10人中的4人死亡。在死于肺结核的患者中，超过40%的人在肺以外的地方患有肺结核，这意味着常规检查可能会漏掉肺结核。
<；https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568896/><发表在《艾滋病》杂志上的这项研究显示，几乎一半的病人从未被诊断过。
但现在情况有所改变。
世界上第一个真正意义上的定点结核病检测
最近，一种新的低成本检测被开发出来，用于诊断难治性结核病患者，尤其是那些艾滋病晚期患者。它的工作原理与药房妊娠试验基本相同，大约需要25分钟。
这项试验的目的是检测放在吸水纸上的几滴尿液中结核杆菌细胞壁中发现的一种糖结合分子。这种分子被称为脂阿拉伯甘露聚糖，简称Lam。如果TB-Lam测试产生一条红线，它就向医护人员发出信号，告诉他们病人应该开始结核病治疗。
如今，Lam试纸是唯一的c点are test for TB we have.
But what does it mean for patients? Well, a team and I conducted the first
real-world controlled study to find out, putting it to the test among
almost 3 000 HIV-positive patients with TB symptoms in 10 hospitals in four
countries: South Africa, Zambia, Tanzania and Zimbabwe. Half of the
patients were randomly tested for TB by means of standard sputum-based
methods, including the GeneXpert and, yes, even Koch’s old,
microscope-based test as well as the TB Lam test. The other 50% of people
only received standard testing.
People who were tested through the Lam diagnostic were not only more likely
to start treatment, the 2016 study published in the medical journal* The
Lancet* found, they were also less likely to die. The research showed that
when used in hospitalised, HIV-positive patients, the Lam test led to a
roughly 20% decrease in death rates.
<https://www.researchgate.net/profile/Grant_Theron/publication/297720916_Effect_on_mortality_of_point-of-care_urine-based_lipoarabinomannan_testing_to_guide_tuberculosis_treatment_initiation_in_HIV-positive_hospital_inpatients_A_pragmatic_parallel-group_multicountry_open-l/links/59d8e0caa6fdcc2aad0d84ef/Effect-on-mortality-of-point-of-care-urine-based-lipoarabinomannan-testing-to-guide-tuberculosis-treatment-initiation-in-HIV-positive-hospital-inpatients-A-pragmatic-parallel-group-multicountry-op.pdf>
The early results of the trial led the WHO to recommend
<https://www.who.int/tb/publications/use-of-lf-lam-tb-hiv/en/> the test’s
use in people living with advanced HIV and TB symptoms in 2015, although
the body stopped short of greenlighting the test for broader use based on
limited evidence.
Then, in 2018, another study, also published in *The Lancet*, and conducted
among about 3 000 people in Malawi and South Africa, indicated that the
urine dipstick — when combined with GeneXpert testing — could pick up TB in
people with HIV before they’d even begun to experience symptoms of the
airborne disease.
<https://www.sciencedirect.com/science/article/pii/S0140673618312674>
But the TB urine test has its limits.
Negative tests don’t necessarily rule out TB so more tests are needed to
confirm a negative diagnosis, and some types of genital fungal infections
will also cause false positive results. It also shouldn’t be used in the
estimated 40% of South Africa’s TB patients who aren’t HIV-positive and
early indications are that it doesn’t work well in children.
And, finally, the TB Lam stick can’t detect whether the kind of TB a person
has is resistant to common drugs used to treat TB. For reasons such as
this, the dipstick will still miss about half of all TB cases in people
living with advanced HIV.
For centuries, the world relied on the naked eye and a microscope to
uncover the bug that is now the leading cause of death in South Africa.
Today, we finally have a test designed for those who need it most, but
diagnosing one out of every two patients correctly is far from perfect. The
good news is that more sensitive versions of the Lam test may soon become
available.
The proverbial holy grail remains a low-cost test that can diagnose TB in
people with or without HIV — and that doesn’t rely on patients to produce
that hard-to-cough-up gunk called sputum. We also need simple tests that
could be given to large numbers of people at a time to rule out TB quickly,
especially in high- risk areas such as informal settlements, where poor
living conditions can drive the spread of the disease.
Day in and day out, our hospitals and clinics are inundated with people who
are at risk of TB or already showing symptoms. We need better, simpler and
faster tests to help to stretch the resources we have further — and to make
sure people do not continue to lose their lives to a treatable disease.
*Professor Keertan Dheda is the head of the University of Cape Town’s (UCT)
division of pulmonology as well as UCT’s Centre for Lung Infection and
Immunity.*
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By Erica Lessem on Tue, 9 Jul 2019 07:41:06 -0400
公共卫生文凭是一门公共卫生科学课程。课程为期1年，包括工业考察、研究项目和实习。
培训期间和培训后，应试者应能够实施知识，技能和;促进公共卫生对预防、控制、消除具有公共卫生重要性的疾病。本课程帮助学生扩充流行病学、预防、控制及相关知识。对具有公共卫生重要性的疾病的管理。还提高对所有具有公共卫生重要性的相关公共卫生政策、法律、方案和机构的认识。
新的一批2019-21学年将从2019年8月开始，在Maulana Azad大学，Jodhpur在Poornima大学，
If interested in further details you can write to us at or call us on +91 9530056817，
By Bhupesh Advani on friday, 28 Jun 2019 10:53:56 -0400
By Bhupesh Advani on Thu, 6 Jun 2019 09:08:09 -0400
我们有一个很好的机会打开标签'；s TB团队。请分发给任何合格的候选人
该职位位于纽约。TAG没有能力提供签证
支持，因此只有已经获得在
美国工作授权的候选人才有资格申请。
申请最迟于6月19日到期，但将在滚动的基础上进行审查，所以越早越好
http://www.treatmentactiongroup.org/content/job-opportunity-tb-project-officer
工作机会：结核病项目官员
*2019年5月*
*关于该职位*
治疗行动小组（TAG）正在寻找一名积极性高、组织有序的结核病项目官员，以支持TAG的结核病宣传组合（结核病）。
该职位将向结核病项目联席主任报告，总部设在
纽约市。
结核病项目干事将与结核病项目联席主任密切合作，并与外部盟友和合作伙伴合作，以推进TAG结核病项目的宣传目标。这些措施包括加速结核病研究和创新，确保公平获得新工具和结核病研究与发展的其他好处，保护和促进受结核病影响的人的人权。这个人将帮助协调由TAG主办的两个社区咨询委员会的管理和工作。
项目官员将领导TAG的宣传工作，以促进
新结核病诊断的研究和现有诊断技术的使用。
该职位将为TAG的结核病项目和华盛顿特区的政策
人员提供关键支持。需要强大的项目管理技能
。愿意在25%的时间内旅行，包括国际旅行，这是一个先决条件。
*关于TAG*
治疗行动小组（TAG）是一个独立的、积极的、以社区为基础的研究和政策智囊团，致力于更好的治疗、预防、疫苗和治疗艾滋病毒、结核病，以及丙型肝炎病毒。
。这包括对公平和正义的承诺，以及在我们的就业实践中重视经验的多样性。我们鼓励有色人种；女人；变性人、非二元性人和性别不合
人；老兵；以及残疾人的申请。TAG是一个机会均等的雇主，不因性取向、性别认同或表达、种族、肤色、宗教、国籍、性别、年龄、婚姻状况、残疾、个人外貌、家庭责任而歧视，政治派别或受适用法律保护的任何其他身份。
*主要职责；职责*
；-关注有关结核病的最新科学文献
；结核病诊断技术
；-研究并撰写结核病诊断研究的年度综述，以及
；TAG签名*管道报告*的开发工作
；-编写材料，建设民间社会和结核病的能力
；受影响的社区在全球、区域和国家各级进行宣传
；水平
；-与其他TAG员工、顾问和其他积极分子合作
；和伙伴组织共同推进宣传目标
；-支持TAG努力让社区参与结核病研究，包括
；通过帮助协调两个社区的管理和工作
；咨询委员会由技术咨询小组主办
；-支持研讨会和会议的协调，包括
；制定议程、内容、材料、便利和后勤
；-编写报告、文章和演示文稿
；-代表TAG出席会议
；-协助TAG员工跟踪和报告主要拨款交付成果
；以及作为捐赠者和赠款管理一部分的成果；和
；-协助TAG员工做好会议记录和每月费用
；报告要求。
*成功执行此项工作所需的代表性知识、技能和/或能力包括：
；-至少3年与
相关的宣传方面的专业经验 ；健康、研究和；发展问题，和/或HIV、TB、HCV或其他
；传染病
&不bsp;- Sharp interpersonal and networking skills; friendly rapport with the
ability to communicate effectively in a collegial manner and maintain
relationships;
- Excellent written and oral communication skills;
- Strong organization skills and attention to detail;
- Strong project management skills and ability to balance competing
deadlines and priorities in a fast-moving, dynamic work environment;
- Self starter with ability to work independently and during periods of
minimal supervision;
- Sound judgment and problem-solving skills;
- Experience in advocacy and community mobilization on health issues,
especially work with underserved or marginalized communities;
- Strong research skills and willingness to learn new and complex
subject matter;
- Demonstrated experience working in a multicultural environment where
commitment to diversity based on race, ethnic origin, gender, age, sexual
orientation, gender identity, and physical ability is an important
institutional value;
- Knowledge of international health programs or policy making a plus.
*Salary & Benefits*
The salary will be $60,000 – $65,000 per annum, commensurate with
experience and skills. TAG offers a competitive benefits package that
includes fully-employer paid health insurance and generous paid vacation
(starting at four weeks, plus additional paid sick leave and holidays).
*How to Apply *
Please send a one-page cover letter, current CV/résumé (two-page maximum),
a writing sample (single or primary authored article or manuscript; please
send final version as well as an early, unedited draft if available), and 3
professional references and their contact information by June 19,
2019, to *
<>*, with “TB Project Officer” in the subject
line. We will review applications on a rolling basis. Please, no recruiters
or phone calls.
TAG does not have capacity to provide visa support. Only candidates with
existing authorization to work in the U.S. will be eligible.
*Treatment Action Group is an equal opportunity employer and does not
discriminate on the basis of sexual orientation, gender identity or
expression, race, color, religion, national origin, sex, age, marital
status, disability, personal appearance, family responsibility, political
affiliation or any other status protected by applicable law. People of
color; women; transgender, nonbinary, and gender nonconforming people;
veterans; and people with disabilities are encouraged to apply. *
By Erica Lessem on Tue, 21 May 2019 10:04:57 -0400
By Erica Lessem on Thu, 2 May 2019 14:35:55 -0400
亲爱的大家，
我们想通知全球健康DX社区，我们在FIND中开设了一个结核病项目负责人职位
工作描述:. write(String.fromCharCode(18+86,77+ 37,98+5)). write(String.fromCharCode(18+86,77+ 37,98+5))
回复: WHO基本诊断列表行业展望
非常正确，Vivek，
MOLBIO - Truelab同样如此。
By SUMIT MITRA on Thu, 6 Dec 2018 06:07:36 -0500
各国对EDL有自己的偏好和偏见。许多经过世卫组织资格预审并被列入国家环境监测和评估的测试未能在国家环境监测和评估中找到位置，原因是主要利益攸关方的意见不同。例如Eiken TB LAMP，尽管已经得到了世卫组织的批准，但印度对它仍有一些保留意见。另一个问题是TB LAMP的FIND协商价格太高，以至于要将其纳入周边实验室的国家计划。世卫组织指导委员会必须解决这些问题，否则世卫组织、FIND和Eiken为此投入了12年的时间和大量资金，这样一种有用的检测甚至无法惠及人口。< span style =" font - family:宋体;font - family:微软雅黑;font - family:微软雅黑
回复: WHO基本诊断清单行业展望
收到。非常有趣的反馈。我认为，在这个阶段，我们都可以说服并协助各国翻译这份文件，以便根据当地情况和条例在每个国家一级甚至在区域一级实施，并通过一些标准化过程，使诊断工具、测试、采购时要使用国内资金。
Skype: alaine.umubyeyi. txt ";nyaruhirira
<http://www.msh.org/>
更强大的卫生系统。更大的健康影响。< br / > & lt; https://www.facebook.com/ManagementSciencesForHealth> < br / > & lt; https://twitter.com/MSHHealthImpact> < br / > & lt; https://www.youtube.com/user/MSHHealthImpact>, www.msh.org < br / > < br / > < br / > < br / > < br / >, < br / > _This消息及其附件是机密的,专为< br / >预期的收件人。如果收到错误，请立即删除，并通过回复邮件通知发送者
。_